Determination of high concentration of glucose by flow injection analysis with immobilized enzyme tube reactor.
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چکیده
منابع مشابه
Routine glucose determination in serum by use of an immobilized glucose dehydrogenase nylon-tube reactor.
We report a method for immobilizing glucose dehydrogenase on the inside surface of nylon tubes to produce an immobilized-enzyme nylon-tube reactor. The glucose dehydrogenase reactor is integrated into the flow system of a continuous-flow analyzer to facilitate routine analysis of serum glucose at 50 samples/h. We compared results with those by the reference hexokinase/glucose-6-phosphate dehydr...
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Strem Chemicals, Inc. 7 Mulliken Way Newburyport, MA 01950-4098 U.S.A. Tel.: (978) 499-1600 Fax: (978) 465-3104 Email: [email protected] Strem Chemicals, Inc. 15, rue de l’Atome Zone Industrielle 67800 BISCHHEIM France Tel.: (33) 03 88 62 52 60 Fax: (33) 03 88 62 26 81 Email: [email protected] Strem Chemicals, Inc. Postfach 1215 77672 KEHL Germany Telefon: 0 78 51/ 7 58 79 Email: info.europe@s...
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We report here the preparation of an immobilized-enzyme nylon-tube reactor containing uricase (EC 1.7.3.3) and the assembly of a flow-through system (Technicon AutoAnalyzer II) for the routine determination of uric acid in serum. Results of these uric acid analyses by use of immobilized uricase, in conjunction with peroxidase (EC 1.11.1.7) and aminophenazone-dichlorophenol in solution, are comp...
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Enzyme-mediated oxidation is of particular interest to synthetic organic chemists. However, the implementation of such systems demands knowledge of enzyme kinetics. Conventionally collecting kinetic data for biocatalytic oxidations is fraught with difficulties such as low oxygen solubility in water and limited oxygen supply. Here, we present a novel method for the collection of such kinetic dat...
متن کاملImmobilized-enzyme rate-determination method for glucose analysis.
We present a rate-determination method for analyzing glucose. A glucose enzyme electrode serves as the sensor and is made by placing a gel-immobilized layer of glucose oxidase over the tip of a Clark-type O2 electrode. The electrode membrane is made of Teflon and is derivatized by etching with a suspension of colloidal sodium metal in organic solvent. The enzyme is coupled to the membrane surfa...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1986
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.35.134